Review



cd4 cd25 regulatory t cell isolation kit  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Miltenyi Biotec cd4 cd25 regulatory t cell isolation kit
    Cd4 Cd25 Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/pm42768744-76-14-23
    Average 96 stars, based on 95 article reviews
    cd4 cd25 regulatory t cell isolation kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Activation Assay:

    Article Title: Serum-derived extracellular vesicles from breast cancer patients contribute to differential regulation of T-cell-mediated immune-escape mechanisms in breast cancer subtypes.
    Article Snippet: For assessment of stimulation of Tregs, naïve T cells were prepared from PBMCs using a Pan T-cell isolation kit (Miltenyi Biotech, Bisley, Surrey, UK 130-096-525) according to the manufacturer’s instructions. .. CD25+ cells were then depleted using CD25 microbeads (Miltenyi Biotec 130-092-983), and CD3+/CD25naïve T cells were then seeded in 96-well U-bottom plates at 2 × 105 cells/well and stimulated with CD3/CD28 activation beads or CD3/ CD28 Dynabeads at a ratio of 1 cell:1 bead plus 0.2 ng/ml IL-2. ..

    Article Title: Serum-derived extracellular vesicles from breast cancer patients contribute to differential regulation of T-cell-mediated immune-escape mechanisms in breast cancer subtypes
    Article Snippet: For assessment of stimulation of Tregs, naïve T cells were prepared from PBMCs using a Pan T-cell isolation kit (Miltenyi Biotech, Bisley, Surrey, UK 130-096-525) according to the manufacturer’s instructions. .. CD25 + cells were then depleted using CD25 microbeads (Miltenyi Biotec 130-092-983), and CD3 + /CD25 - naïve T cells were then seeded in 96-well U-bottom plates at 2 × 10 5 cells/well and stimulated with CD3/CD28 activation beads or CD3/CD28 Dynabeads at a ratio of 1 cell:1 bead plus 0.2 ng/ml IL-2. ..

    Lysis:

    Article Title: Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axis.
    Article Snippet: TGFbR1 inhibitor treatment Leukopaks from healthy donors were obtained from StemExpress, BioIVT, or AllCells, with their full consent, under the approval of Institutional Review Board obtained by these companies. .. PBMCs were treated with ACK lysis to remove residual red blood cells, followed by positive selection for CD25+ cells (Miltenyi Biotec, 130092983). .. These enriched CD25+ cells were sorted by FASC to isolate CD4+CD25+CD127−CD45RO- naïve Treg cells or CD4+CD25+CD127−CD45RO+ effector Treg cells on FACSAriaTM II or FACSAriaTM Fusion (BD Biosciences) with the indicated antibodies.

    Article Title: Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axis
    Article Snippet: Leukopaks from healthy donors were obtained from StemExpress, BioIVT, or AllCells, with their full consent, under the approval of Institutional Review Board obtained by these companies. .. PBMCs were treated with ACK lysis to remove residual red blood cells, followed by positive selection for CD25 + cells (Miltenyi Biotec, 130092983). .. These enriched CD25 + cells were sorted by FASC to isolate CD4 + CD25 + CD127 − CD45RO - naïve Treg cells or CD4 + CD25 + CD127 − CD45RO + effector Treg cells on FACSAriaTM II or FACSAriaTM Fusion (BD Biosciences) with the indicated antibodies.

    Selection:

    Article Title: Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axis.
    Article Snippet: TGFbR1 inhibitor treatment Leukopaks from healthy donors were obtained from StemExpress, BioIVT, or AllCells, with their full consent, under the approval of Institutional Review Board obtained by these companies. .. PBMCs were treated with ACK lysis to remove residual red blood cells, followed by positive selection for CD25+ cells (Miltenyi Biotec, 130092983). .. These enriched CD25+ cells were sorted by FASC to isolate CD4+CD25+CD127−CD45RO- naïve Treg cells or CD4+CD25+CD127−CD45RO+ effector Treg cells on FACSAriaTM II or FACSAriaTM Fusion (BD Biosciences) with the indicated antibodies.

    Article Title: Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axis
    Article Snippet: Leukopaks from healthy donors were obtained from StemExpress, BioIVT, or AllCells, with their full consent, under the approval of Institutional Review Board obtained by these companies. .. PBMCs were treated with ACK lysis to remove residual red blood cells, followed by positive selection for CD25 + cells (Miltenyi Biotec, 130092983). .. These enriched CD25 + cells were sorted by FASC to isolate CD4 + CD25 + CD127 − CD45RO - naïve Treg cells or CD4 + CD25 + CD127 − CD45RO + effector Treg cells on FACSAriaTM II or FACSAriaTM Fusion (BD Biosciences) with the indicated antibodies.

    Isolation:

    Article Title: Controlled transgene expression in regulatory t cells
    Article Snippet: .. CD4+ T cells are isolated from a human donor via RosetteSepTM (STEMCELL Technologies, 15062) and enriched for CD25+ cells (Miltenyi Biotec, 130-092-983) prior to sorting live CD4+CD25hiCD127lo Tregs or CD4+CD127loCD25hiCD45RA+Tregs using a MoFlo® AstriosTM (Beckman Coulter) or FACSAriaTM II (BD Biosciences). .. Sorted Tregs may be stimulated with L cells and anti-CD3 monoclonal antibody (e.g., OKT3, UBC AbLab; 100 ng/ml) in ImmunoCultTM-XF T cell expansion media (STEMCELL Technologies, 10981) with 1000 U/ml IL-2 (Proleukin) as described in MacDonald et al., J Clin Invest. (2016) 126(4):1413-24).

    Incubation:

    Article Title: Vasoactive intestinal peptide release from microparticles
    Article Snippet: Following RBC lysis, CD4+ cell isolation are performed using the CD4 negative isolation kit (Invitrogen) as per the manufacturer's instructions. .. To enrich for CD25− cells, CD4+ cells are incubated with anti-mouse CD25-PE antibody (eBioscience) followed by addition of anti-PE microbeads (Miltenyi). ..

    Labeling:

    Article Title: Compositions and methods of hematopoietic stem cell transplants
    Article Snippet: .. The CD25+ cells recovered by enrichment of CD25+ and 6B11V cells in Step B were volume adjusted for labeling with PerCP-conjugated mouse anti-human CD4 (Miltenyi Biotec) and APC-conjugated mouse anti-human CD127 monoclonal antibody reagents (Miltenyi Biotec), and additional CD25 PE (Miltenyi Biotec) and Streptavidin PE-Cy7. ..

    Article Title: Compositions and methods of hematopoietic stem cell transplants
    Article Snippet: Step D: Treg and iNKT cells were further purified by cell sorting using a BD FACS ARIA. .. The CD25+ cells recovered by enrichment of CD25+ and CD1d (glycolipid loaded) tetramer+ cells in Step B were volume adjusted for labeling with PerCP-conjugated mouse anti-human CD4 and APC-conjugated mouse anti-human CD127 monoclonal antibody reagents and additional CD25 PE (Miltenyi Biotec) and CD1d (glycolipid loaded) tetramer PE-Cy7. ..



    Similar Products

    cd4 cd25 regulatory t cell isolation kit  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd4 cd25 regulatory t cell isolation kit
    Cd4 Cd25 Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/pm42768744-76-14-23
    Average 96 stars, based on 1 article reviews
    cd4 cd25 regulatory t cell isolation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    human cd4 cd25 regulatory t cell isolation kit  (Miltenyi Biotec)
    96
    Miltenyi Biotec human cd4 cd25 regulatory t cell isolation kit
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Human Cd4 Cd25 Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/pmc13627347-100-19-29
    Average 96 stars, based on 1 article reviews
    human cd4 cd25 regulatory t cell isolation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    cd4+cd25+ regulatory t cell isolation kit, mouse  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd4+cd25+ regulatory t cell isolation kit, mouse
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Cd4+Cd25+ Regulatory T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/custom%40130-091-041%4042480527
    Average 96 stars, based on 1 article reviews
    cd4+cd25+ regulatory t cell isolation kit, mouse - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    purification  (Miltenyi Biotec)
    96
    Miltenyi Biotec purification
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Purification, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/pmc13290390-159-2-5
    Average 96 stars, based on 1 article reviews
    purification - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    two step regulatory t cell isolation kit  (Miltenyi Biotec)
    96
    Miltenyi Biotec two step regulatory t cell isolation kit
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Two Step Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/10__1158_slash_2767___9764__crc___26___0207-100-25-32
    Average 96 stars, based on 1 article reviews
    two step regulatory t cell isolation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    mouse cd4 cd25 regulatory t cell isolation kit  (Miltenyi Biotec)
    96
    Miltenyi Biotec mouse cd4 cd25 regulatory t cell isolation kit
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Mouse Cd4 Cd25 Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/pm42242573-58-14-20
    Average 96 stars, based on 1 article reviews
    mouse cd4 cd25 regulatory t cell isolation kit - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    cd4 cd25 cd127dim  (Miltenyi Biotec)
    95
    Miltenyi Biotec cd4 cd25 cd127dim
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Cd4 Cd25 Cd127dim, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2BCD127dim%2F-+Regulatory+T+Cell+Isolation+Kit+II%2C+human/pm42212623-69-5-14
    Average 95 stars, based on 1 article reviews
    cd4 cd25 cd127dim - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    regulatory t cell isolation kit ii  (Miltenyi Biotec)
    95
    Miltenyi Biotec regulatory t cell isolation kit ii
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Regulatory T Cell Isolation Kit Ii, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cells/CD4%2BCD25%2BCD127dim%2F-+Regulatory+T+Cell+Isolation+Kit+II%2C+human/pmc13318110-264-20-26
    Average 95 stars, based on 1 article reviews
    regulatory t cell isolation kit ii - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Control, Lysis, Flow Cytometry, Staining, In Vitro, Isolation, Incubation, Expressing, Comparison

    PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Analysis, Activation Assay, Immunohistochemistry, Expressing

    PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Tissue, Microinjection, Injection, Staining, Analysis, Expressing, Activity Assay